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epidermal growth factor  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec epidermal growth factor
    Epidermal Growth Factor, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+epidermal+growth+factor/Human+EGF%2C+premium+grade/pm41832602-60-31-34
    Average 95 stars, based on 68 article reviews
    epidermal growth factor - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Compounds with biguanidyl radical and uses thereof
    Article Snippet: Copper chloride (CuCl2, 459097, Sigma Aldrich), Ferrostatin-1 (Fer-1, SML0583, Sigma Aldrich, 10 μM for 72 h), human epidermal growth factor (EGF, 130-093-825, Miltenyi Biotech, 100 ng/ml for 96 h (plus 72 h for cell viability measurements), carbonyl cyanide mchlorophenylhydrazone (CCCP, 50 μM), Metformin (1,1-Dimethylbiguanide hydrochloride, J63361, Alfa Aesar, 12 mM unless stated otherwise), Metforminyn (Met, in-house drug, 2 mM unless stated otherwise), Necrostatin-1 (N9037, Sigma Aldrich, 20 μM for 72 h), Turn-off Cu(II) probe (10 μM for 1 h), Turn-off Fe(II) probe (1 μM for 1 h), Z-VAD-FMK (550377, BD Biosciences, 50 μM for 72 h).

    Cell Culture:

    Article Title: Epigenomic reprogramming of therapy-resistant circulating tumor cells in colon cancer
    Article Snippet: .. All CTC lines were cultured at 37°C and 5% CO 2 in ultra-low attachment flasks (Corning) with RPMI-1640 medium (Merck) supplemented with 10% fetal bovine serum (Merck), 1% penicillin/streptomycin solution (Gibco), 1% L-glutamine (Merck), 1% of Insulin-Transferrin-Selenium (ITS-G) (Gibco), basic human fibroblast growth factor (Miltenyi Biotec) at a final concentration of 10 ng/mL and human epidermal growth factor (Miltenyi Biotec) at a final concentration of 20 ng/mL. .. Total genomic DNA was isolated from the nine CTC lines using a standard high salt method based on SDS/proteinase K. The isolated DNA was treated with RNase A (Qiagen) following the manufacturer’s recommendations and stored at −80°C until analysis.

    Article Title: A 3D multi-cellular tissue model of the human omentum to study the formation of ovarian cancer metastasis.
    Article Snippet: .. MeT-5A: The human mesothelial cell line MeT-5A was purchased from American Type Culture Collection (ATCC® CRL-9444TM, LGC Standards, Germany) and cultured in Medium 199 (M199, 31150022, Gibco, Thermo Fisher Scientific, Switzerland) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, Switzerland), 1% (v/v) penicillin/streptomycin (P/S, Gibco, Thermo Fisher Scientific, Switzerland), 20 mM HEPES (H0887, Sigma-Aldrich, Switzerland), 400 nM hydrocortisone (H0888, Sigma-Aldrich, Switzerland), 870 nM insulin (I0516, Sigma-Aldrich, Switzerland), 0.3869 mg L− 1 sterile filtered selenous acid (211176, Sigma-Aldrich, Switzerland), 3.3 nM human epidermal growth factor (EGF, 130-097- 749, Miltenyi Biotec Inc., Germany) and 1:1000 Trace Elements B (25- 022-CI, CorningTM, Thermo Fisher Scientific, Switzerland). .. MRC-5: The human fibroblast cell line was purchased from ATCC® (CCL-171TM, LGC Standards, Germany) and cultured in Minimum Essential Media (MEM, 21090–022, Gibco, Thermo Fisher Scientific, Switzerland) supplemented with 10% (v/v) FBS, 1% (v/v) L-glutamine (L-Glu, 25030–024, Gibco, Thermo Fisher Scientific, Switzerland), 1% (v/v) P/S, and 1% (v/v) non-essential amino acids (11140–035, Gibco, Thermo Fisher Scientific, Switzerland).

    Article Title: High-Resolution In Situ High-Content Imaging of 3D-Bioprinted Single Breast Cancer Spheroids for Advanced Quantification of Benzo( a )pyrene Carcinogen-Induced Breast Cancer Stem Cells.
    Article Snippet: Cancer stem cells (CSCs), also known as tumor-initiating cells, are critically correlated with carcinogenesis and are strongly affected by the environmental factors.. Environmental carcinogens, such as benzo(a)pyrene (BaP), are associated with the overproduction of CSCs in various types of cancers, including breast cancer.. In this report, we present a sophisticated 3D breast cancer spheroid model for the direct identification and quantitative determination of CSCs induced by carcinogens within intact 3D spheroids.

    Article Title: Improving Localized Radiotherapy for Glioblastoma via Small Molecule Inhibition of KIF11.
    Article Snippet: .. Cells were cultured at 37 ◦C at 5% CO2 in Neurobasal media (minus phenol red; Gibco, Grand Island, NY, USA) supplemented with B27 (minus Vitamin A; Gibco), human fibroblast growth factor-2 (10 ng/mL; Miltenyi Biotec, Bergisch Gladbach, Germany), human epidermal growth factor (10 ng/mL; Miltenyi Biotec), L-glutamine (2 mM; Gibco), sodium pyruvate (1 mM; Gibco), and penicillin/streptomycin (100 I.U./mL/100 μg/mL; Gibco). .. Cells plated adherently were on Geltrex LDEV-Free hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Gibco), whereas in vivo studies were performed with cells grown in suspension as tumorspheres before dissociation and cell counting prior to implantation.

    Concentration Assay:

    Article Title: Epigenomic reprogramming of therapy-resistant circulating tumor cells in colon cancer
    Article Snippet: .. All CTC lines were cultured at 37°C and 5% CO 2 in ultra-low attachment flasks (Corning) with RPMI-1640 medium (Merck) supplemented with 10% fetal bovine serum (Merck), 1% penicillin/streptomycin solution (Gibco), 1% L-glutamine (Merck), 1% of Insulin-Transferrin-Selenium (ITS-G) (Gibco), basic human fibroblast growth factor (Miltenyi Biotec) at a final concentration of 10 ng/mL and human epidermal growth factor (Miltenyi Biotec) at a final concentration of 20 ng/mL. .. Total genomic DNA was isolated from the nine CTC lines using a standard high salt method based on SDS/proteinase K. The isolated DNA was treated with RNase A (Qiagen) following the manufacturer’s recommendations and stored at −80°C until analysis.

    Suspension:

    Article Title: Modulating voltage-gated sodium channels to enhance differentiation and sensitize glioblastoma cells to chemotherapy.
    Article Snippet: .. GSCs from Florio’s laboratory were cultivated in suspension as spheroid aggregates in stem cell-permissive medium (1:1 DMEM High Glucose and Ham’s F-12 Nutrient Mix-GlutaMAXTM - GibcoTM, Thermo Fisher Scientific), enriched with B27 supplement (GibcoTM, Thermo Fisher Scientific), 10 ng/ml human basic fibroblast growth factor (bFGF, Miltenyi Biotec), and 20 ng/ml human epidermal growth factor (EGF, Miltenyi Biotec). ..

    Article Title: Modulating voltage-gated sodium channels to enhance differentiation and sensitize glioblastoma cells to chemotherapy
    Article Snippet: .. GSCs from Florio’s laboratory were cultivated in suspension as spheroid aggregates in stem cell-permissive medium (1:1 DMEM High Glucose and Ham’s F-12 Nutrient Mix-GlutaMAXTM - GibcoTM, Thermo Fisher Scientific), enriched with B27 supplement (GibcoTM, Thermo Fisher Scientific), 10 ng/ml human basic fibroblast growth factor (bFGF, Miltenyi Biotec), and 20 ng/ml human epidermal growth factor (EGF, Miltenyi Biotec). ..

    Sterility:

    Article Title: A 3D multi-cellular tissue model of the human omentum to study the formation of ovarian cancer metastasis.
    Article Snippet: .. MeT-5A: The human mesothelial cell line MeT-5A was purchased from American Type Culture Collection (ATCC® CRL-9444TM, LGC Standards, Germany) and cultured in Medium 199 (M199, 31150022, Gibco, Thermo Fisher Scientific, Switzerland) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, Switzerland), 1% (v/v) penicillin/streptomycin (P/S, Gibco, Thermo Fisher Scientific, Switzerland), 20 mM HEPES (H0887, Sigma-Aldrich, Switzerland), 400 nM hydrocortisone (H0888, Sigma-Aldrich, Switzerland), 870 nM insulin (I0516, Sigma-Aldrich, Switzerland), 0.3869 mg L− 1 sterile filtered selenous acid (211176, Sigma-Aldrich, Switzerland), 3.3 nM human epidermal growth factor (EGF, 130-097- 749, Miltenyi Biotec Inc., Germany) and 1:1000 Trace Elements B (25- 022-CI, CorningTM, Thermo Fisher Scientific, Switzerland). .. MRC-5: The human fibroblast cell line was purchased from ATCC® (CCL-171TM, LGC Standards, Germany) and cultured in Minimum Essential Media (MEM, 21090–022, Gibco, Thermo Fisher Scientific, Switzerland) supplemented with 10% (v/v) FBS, 1% (v/v) L-glutamine (L-Glu, 25030–024, Gibco, Thermo Fisher Scientific, Switzerland), 1% (v/v) P/S, and 1% (v/v) non-essential amino acids (11140–035, Gibco, Thermo Fisher Scientific, Switzerland).

    Construct:

    Article Title: High-Resolution In Situ High-Content Imaging of 3D-Bioprinted Single Breast Cancer Spheroids for Advanced Quantification of Benzo( a )pyrene Carcinogen-Induced Breast Cancer Stem Cells.
    Article Snippet: Cancer stem cells (CSCs), also known as tumor-initiating cells, are critically correlated with carcinogenesis and are strongly affected by the environmental factors.. Environmental carcinogens, such as benzo(a)pyrene (BaP), are associated with the overproduction of CSCs in various types of cancers, including breast cancer.. In this report, we present a sophisticated 3D breast cancer spheroid model for the direct identification and quantitative determination of CSCs induced by carcinogens within intact 3D spheroids.

    Knock-Out:

    Article Title: Induction of human trophoblast stem cells.
    Article Snippet: Cell reprogramming has allowed unprecedented access to human development, from virtually any genome.. However, reprogramming yields pluripotent stem cells that can differentiate into all cells that form a fetus, but not extraembryonic annexes.. Therefore, a cellular model allowing study of placental development from a broad genomic repertoire is lacking.

    Clinical Proteomics:

    Article Title: Induction of human trophoblast stem cells.
    Article Snippet: Cell reprogramming has allowed unprecedented access to human development, from virtually any genome.. However, reprogramming yields pluripotent stem cells that can differentiate into all cells that form a fetus, but not extraembryonic annexes.. Therefore, a cellular model allowing study of placental development from a broad genomic repertoire is lacking.



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